the bumbling biochemist

@UChzTIByeodToYsKiNiwKQrw - 36.3K subscribers

Assistant Professor of Biochemistry at Loyola Marymount University trying to make biochemistry fun and accessible for all. I aim for lots of detail without lots of jargon (or at least with jargon explained). I do not monetize any of my content. All of it is freely provided out of an effort to "pay it forward." Much more on my blog. I sincerely hope it helps you!

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Protein purification evidence collection – save as you go, so if you lose protein, you'll know where

Protein purification evidence collection – save as you go, so if you lose protein, you'll know where the bumbling biochemist

271 views - 1 day ago

Mass Photometry: a cool newish technique to keep an eye on

Mass Photometry: a cool newish technique to keep an eye on the bumbling biochemist

407 views - 3 days ago

Some resources for scientists in training

Some resources for scientists in training the bumbling biochemist

357 views - 4 days ago

Basic, applied, translational & clinical research: What's the diff? And why's basic research useful?

Basic, applied, translational & clinical research: What's the diff? And why's basic research useful? the bumbling biochemist

490 views - 1 week ago

Antibody therapeutics overview – monoclonals, bispecifics, ADCs, etc.

Antibody therapeutics overview – monoclonals, bispecifics, ADCs, etc. the bumbling biochemist

443 views - 1 week ago

Biochemistry trends, the value of protein biochemistry training, & other thoughts from #ASBMB26

Biochemistry trends, the value of protein biochemistry training, & other thoughts from #ASBMB26 the bumbling biochemist

508 views - 1 week ago

"Statistically significant" doesn't mean important, meaningful, or even true! & can be misleading

"Statistically significant" doesn't mean important, meaningful, or even true! & can be misleading the bumbling biochemist

2.9K views - 1 week ago

Nonfiction science book recommendations involving insulin and/or diabetes

Nonfiction science book recommendations involving insulin and/or diabetes the bumbling biochemist

267 views - 2 weeks ago

Journal Impact Factor (JIF): What it is and why it’s a problem (a bit of a rant, sorry...)

Journal Impact Factor (JIF): What it is and why it’s a problem (a bit of a rant, sorry...) the bumbling biochemist

700 views - 2 weeks ago

Off-target and on-target activity/effects & strategies wrt CRISPR, pharmaceuticals, & PCR

Off-target and on-target activity/effects & strategies wrt CRISPR, pharmaceuticals, & PCR the bumbling biochemist

275 views - 2 weeks ago

A practical guide to scientific figures and their legends/captions (making, writing & reading them)

A practical guide to scientific figures and their legends/captions (making, writing & reading them) the bumbling biochemist

641 views - 2 weeks ago

Scientific conferences: What to expect, how to prepare, & how to make the most of your time there!

Scientific conferences: What to expect, how to prepare, & how to make the most of your time there! the bumbling biochemist

393 views - 2 weeks ago

You can improve the linearity & sensitivity of a Bradford assay by taking 595(or 590)/450 ratio

You can improve the linearity & sensitivity of a Bradford assay by taking 595(or 590)/450 ratio the bumbling biochemist

402 views - 2 weeks ago

Overview of protein concentration determination methods (Bradford, BCA, UV280, etc.) – pros & cons

Overview of protein concentration determination methods (Bradford, BCA, UV280, etc.) – pros & cons the bumbling biochemist

745 views - 2 weeks ago

Gradient elution strategies for protein chromatography (esp. His-tag chromatography via Ni-affinity)

Gradient elution strategies for protein chromatography (esp. His-tag chromatography via Ni-affinity) the bumbling biochemist

447 views - 3 weeks ago

Don't be fooled by imidazole! It absorbs UV280, just like protein – on chromatogram it'll be seen!

Don't be fooled by imidazole! It absorbs UV280, just like protein – on chromatogram it'll be seen! the bumbling biochemist

754 views - 3 weeks ago

The bumbling biochemist’s favorite under-appreciated lab equipment

The bumbling biochemist’s favorite under-appreciated lab equipment the bumbling biochemist

355 views - 3 weeks ago

Unintentional error: Avoiding common experimental artifacts (especially batch & positional effects)

Unintentional error: Avoiding common experimental artifacts (especially batch & positional effects) the bumbling biochemist

2.3K views - 3 weeks ago

Book recs involving pharma companies acting badly (or just selfishly) &/or scientific fraud

Book recs involving pharma companies acting badly (or just selfishly) &/or scientific fraud the bumbling biochemist

747 views - 3 weeks ago

Techniques for buffer exchange (Desalting columns, Dialysis & Diafiltration): Pros, cons & going-ons

Techniques for buffer exchange (Desalting columns, Dialysis & Diafiltration): Pros, cons & going-ons the bumbling biochemist

584 views - 3 weeks ago

Reusing spin concentrators (centrifugal filters)

Reusing spin concentrators (centrifugal filters) the bumbling biochemist

377 views - 3 weeks ago

Flash freezing purified protein

Flash freezing purified protein the bumbling biochemist

834 views - 4 weeks ago

Practical tips for using spin concentrators (centrifugal filters), esp. for protein concentrating

Practical tips for using spin concentrators (centrifugal filters), esp. for protein concentrating the bumbling biochemist

679 views - 4 weeks ago

"Back up" your clones! What to do after you confirm your plasmid sequence

"Back up" your clones! What to do after you confirm your plasmid sequence the bumbling biochemist

398 views - 4 weeks ago

Whole plasmid sequencing with nanopore technology (ONT)

Whole plasmid sequencing with nanopore technology (ONT) the bumbling biochemist

583 views - 4 weeks ago

Key things to know about protein purification & “pro”-tein purification tips

Key things to know about protein purification & “pro”-tein purification tips the bumbling biochemist

436 views - 4 weeks ago

Cloning cells (e.g. DH5α) vs. expression cells (e.g. BL21) & features of each

Cloning cells (e.g. DH5α) vs. expression cells (e.g. BL21) & features of each the bumbling biochemist

545 views - 4 weeks ago

Demystifying (and de-mythifying) grad school – things to know about what it is and isn’t

Demystifying (and de-mythifying) grad school – things to know about what it is and isn’t the bumbling biochemist

574 views - 4 weeks ago

Scientific writing is an iterative, often collaborative & back-and-forth, process–here's some advice

Scientific writing is an iterative, often collaborative & back-and-forth, process–here's some advice the bumbling biochemist

491 views - 4 weeks ago

Your LB/agar plate has colonies. Yay! Now what?

Your LB/agar plate has colonies. Yay! Now what? the bumbling biochemist

390 views - 4 weeks ago

Attaching HiTrap and other small columns to an AKTA column valve

Attaching HiTrap and other small columns to an AKTA column valve the bumbling biochemist

662 views - 4 weeks ago

You can skip the post-transformation outgrowth if using ampicillin as a selectable marker Here's why

You can skip the post-transformation outgrowth if using ampicillin as a selectable marker Here's why the bumbling biochemist

399 views - 4 weeks ago

Overview of Q5 SDM: Site-Directed Mutagenesis using the NEB Q5 SDM Kit

Overview of Q5 SDM: Site-Directed Mutagenesis using the NEB Q5 SDM Kit the bumbling biochemist

443 views - 4 weeks ago

HDX-MS (Hydrogen Deuterium eXchange - Mass Spectrometry) –fundamentals

HDX-MS (Hydrogen Deuterium eXchange - Mass Spectrometry) –fundamentals the bumbling biochemist

833 views - 4 weeks ago

Chromatography column volumes (CVs) & how to calculate them

Chromatography column volumes (CVs) & how to calculate them the bumbling biochemist

324 views - 4 weeks ago

Tips for improving your resolution in SEC (Size Exclusion Chromatography)(aka gel filtration)

Tips for improving your resolution in SEC (Size Exclusion Chromatography)(aka gel filtration) the bumbling biochemist

386 views - 4 weeks ago

ResolutIon in protein chromatography – what it is & how to get it: selectivity & efficiency

ResolutIon in protein chromatography – what it is & how to get it: selectivity & efficiency the bumbling biochemist

406 views - 4 weeks ago

Differential Scanning Fluorimetry/Fluorescence Thermal Shift/Melt (lots of names . . .)

Differential Scanning Fluorimetry/Fluorescence Thermal Shift/Melt (lots of names . . .) the bumbling biochemist

527 views - 4 weeks ago

Beyond AlphaFold: The future of structural biology is likely "dynamic" (molecular dynamics, etc.)

Beyond AlphaFold: The future of structural biology is likely "dynamic" (molecular dynamics, etc.) the bumbling biochemist

659 views - 4 weeks ago

Who's who (position-wise) in academic science labs: PI, grad students, postdocs, lab techs, etc.

Who's who (position-wise) in academic science labs: PI, grad students, postdocs, lab techs, etc. the bumbling biochemist

725 views - 4 weeks ago

Recombinant protein expression systems & trying to get expression to work in bacterial cells

Recombinant protein expression systems & trying to get expression to work in bacterial cells the bumbling biochemist

541 views - 4 weeks ago

Inducible overexpression of protein in bacteria using autoinduction media or IPTG & the lac promoter

Inducible overexpression of protein in bacteria using autoinduction media or IPTG & the lac promoter the bumbling biochemist

573 views - 4 weeks ago

Aptamers and a quick comparison of small nucleic acid tools/therapeutics (RNAi-based & ASOs)

Aptamers and a quick comparison of small nucleic acid tools/therapeutics (RNAi-based & ASOs) the bumbling biochemist

438 views - 4 weeks ago

Gene-related jargon: exons, introns, UTRs, pre-mRNA, mature mRNA, CDS (coding sequence), & cDNA

Gene-related jargon: exons, introns, UTRs, pre-mRNA, mature mRNA, CDS (coding sequence), & cDNA the bumbling biochemist

424 views - 1 month ago

Preprints, Peer Review, and Open Access science journals & articles

Preprints, Peer Review, and Open Access science journals & articles the bumbling biochemist

213 views - 1 month ago

Things to look for in a vector plasmid

Things to look for in a vector plasmid the bumbling biochemist

573 views - 1 month ago

High-throughput vs. lower-throughput experimental methods

High-throughput vs. lower-throughput experimental methods the bumbling biochemist

242 views - 1 month ago

Ways to measure "expression" of genes and proteins: a quick overview of techniques

Ways to measure "expression" of genes and proteins: a quick overview of techniques the bumbling biochemist

409 views - 1 month ago

Hit the lab ground running! Some suggestions for starting in biochemistry-related fields

Hit the lab ground running! Some suggestions for starting in biochemistry-related fields the bumbling biochemist

345 views - 1 month ago

Proteoforms versus isoforms and the challenges of measuring them

Proteoforms versus isoforms and the challenges of measuring them the bumbling biochemist

323 views - 1 month ago

Software tools and databases for working with proteins

Software tools and databases for working with proteins the bumbling biochemist

298 views - 1 month ago

Basic nomenclature of atom identifiers for proteins in PDB (and related) files

Basic nomenclature of atom identifiers for proteins in PDB (and related) files the bumbling biochemist

233 views - 1 month ago

Converting from .cif to .pdb files in PyMOL and ChimeraX

Converting from .cif to .pdb files in PyMOL and ChimeraX the bumbling biochemist

390 views - 1 month ago

Genetic "knock-down" via RNAi (siRNA, shRNA, miR-451 analogs, etc.)

Genetic "knock-down" via RNAi (siRNA, shRNA, miR-451 analogs, etc.) the bumbling biochemist

444 views - 1 month ago

RNAi animation (not narrated)

RNAi animation (not narrated) the bumbling biochemist

151 views - 1 month ago

Interpreting AlphaFold predictions and confidence indicators

Interpreting AlphaFold predictions and confidence indicators the bumbling biochemist

618 views - 1 month ago

Quick overview of techniques to target molecules at multiple levels: gene, pre-mRNA, mRNA, & protein

Quick overview of techniques to target molecules at multiple levels: gene, pre-mRNA, mRNA, & protein the bumbling biochemist

301 views - 1 month ago

Helpful tool for generating protein topology & domain diagrams & investigating interfaces: PDBSum

Helpful tool for generating protein topology & domain diagrams & investigating interfaces: PDBSum the bumbling biochemist

872 views - 3 months ago

Strategy for metabolic “accounting” problems (# of ATP, NADH, etc.) with worked practice problems

Strategy for metabolic “accounting” problems (# of ATP, NADH, etc.) with worked practice problems the bumbling biochemist

342 views - 3 months ago

Anaplerotic reactions to TCA put carbons in if you take 'em away (or you can't bring in acetyl-CoA!)

Anaplerotic reactions to TCA put carbons in if you take 'em away (or you can't bring in acetyl-CoA!) the bumbling biochemist

290 views - 3 months ago